Objective:  The aim of the present work is to study the protective effect of some antioxidants and trace elements against the hazardous effects of carbon tetrachloride (CCl₄₎ on hamsters for the possibility of further application on humans. Methods:  One hundred twenty hamsters weighing 104-128g were divided into 13 groups as follows: 1-Negative Control group fed standard diet, 2-positive control group given carbon tetrachloride (CCl₄ ) only , 3-CCl₄ + zinc, 4 - CCl₄ + β-carotene,5- CCl₄ + α-tocopherol,6- CCl₄ + selenium, 7-CCl₄ + vitamin C,8- CCl₄ + zinc + β - carotene, 9-CCl₄ + zinc + vitamin C , 10-CCl₄ + zinc + α-tocopheral,11-CCl₄ + selenium + β-carotene, 12-CCl₄ + selenium + vitamin C and 13-CCl₄ + selenium + α-tocopheral.  Results: Carbon tetrachloride (CCl₄) has a direct toxic effect on liver and kidney. Depending on biochemical results, the more antioxidants of liver protection against CCl₄ toxicity are α tocopherol, followed by selenium, selenium + β - carotene, vitamin C, zinc + β – carotene and selenium + α tocopherol. The more antioxidant for kidney protection against CCl₄ toxicity is β –carotene followed by selenium, zinc + β – carotene, zinc + α tocopherol, and zinc + vitamin C. Conclusion: Trace elements should not be given individually especially zinc or selenium as deleterious effects, in spite of its protective effect.