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17386

Optimization of fibrinase productivity from Actinomycetes

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Last updated: 03 Jan 2025

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Abstract

Background: Fibrinolytic enzymes that dissolve blood clots and show promise for thrombosis therapy have been successfully identified from various sources. A wide range of microorganisms has been screened for their fibrinolytic properties. A fibrinolytic protease has been isolated from Streptomyces violaceoruber and Streptomyces spiroverticillatus culture filtrate. Methods : The purification procedure involved ammonium sulphate fractionation, dialysis, calcium phosphate gel purification and gel filtration on Sephadex G-100. By using native polyacrylamide gel electrophoresis (Native PAGE) to determine molecular weight of the enzyme. Results : The optimum temperature for the high production of fibrinase from S. violaceoruber was 30oC and from S. spiroverticillatus was 35oC and the optimum pH was 9.0. The best incubation period is 6 days. The incorporation of lactose as carbon source, yeast extract as nitrogen source and MnCl2 to culture media highly increased the production of fibrinase from the two species. The molecular weight was about 30 KDa. Conclusion: It exhibited fibrinolytic enzyme activity. In vitro studies revealed that fibrinase dissolves clots made by blood.

DOI

10.21608/ejhm.2010.17386

Keywords

Key words: Fibrinase, Fibrinolytic enzyme, fibrin clots

Authors

First Name

Habib

Last Name

S. A

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Affiliation

ChemistryDepartment(BiochemistryDivision),FacultyofScience(Damietta), Mansoura University, Egypt.

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First Name

Abo doubara

Last Name

M. I

MiddleName

-

Affiliation

BotanyDepartment,FacultyofScience(Damietta),MansouraUniversity,Egypt.

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First Name

Abdel-Malak Camelia.

Last Name

A

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-

Affiliation

ChemistryDepartment(BiochemistryDivision),FacultyofScience(Damietta), Mansoura University, Egypt.

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Orcid

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First Name

Badawy Rasha.

Last Name

M.

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-

Affiliation

ChemistryDepartment(BiochemistryDivision),FacultyofScience(Damietta), Mansoura University, Egypt.

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Volume

40

Article Issue

1

Related Issue

3652

Issue Date

2010-07-01

Receive Date

2018-10-23

Publish Date

2010-07-01

Page Start

375

Page End

388

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 40_Issue 1_Pages 375-388.pdf

PDF . 556.9kB

Link

https://ejhm.journals.ekb.eg/article_17386.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=17386

Order

7

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Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Optimization of fibrinase productivity from Actinomycetes

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Article

Created At

22 Jan 2023