Beta
ManageSearchChatDiscover
17380

Identification and Characterization of Nuclear Matrix Protein(s) and assessment of its usefullness for early diagnosis of bladder cancer

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Background: the highest incidence rates of bladder cancer are generally found in industrially developed countries, particularly North America and
Western Europe, and areas associated with endemic schistosomiasis, including parts of Africa and the Middle East . The appropriate treatment of patients with bladder cancer mandates early detection and regular follow up for recurrences . Currently, cystoscopy is the standard method for diagnosing and monitoring bladder cancer recurrence, but it is an invasive and relatively costly technique, and may sometimes be inconclusive, particularly in cases of cystitis . Patients and methods: western blot and specific immunoglobulin-G antibody were used to identify the urinary NMP marker. Urine samples from 123 patients
with bladder cancer and 50 controls were evaluated using the developed SDS-PAGE, Western blot and ELISA. Results: the NMP marker was identified in the urine of
patients with bladder cancer at 52 kDa (NMP-
52) by SDS-PAGE, Western blot and ELISA. In addition, the NMP-52 tumor marker was not detected in the urine
of patients . Conclusion: detecting the urinary NMP-52 marker using SDS-PAGE, Western blot and ELISA, would be helpful in the rapid diagnosis of bladder cancer.  

DOI

10.21608/ejhm.2010.17380

Keywords

bladder, Tumor, marker, NMP, urine, Diagnosis, SDS - PAGE, western blot, ELISA

Authors

First Name

Abdelfattah M.

Last Name

Attallah

MiddleName

-

Affiliation

R & D Department, Biotechnology Research Center, New Damietta City, Egypt

Email

-

City

-

Orcid

-

First Name

Seham E.

Last Name

Mansy

MiddleName

-

Affiliation

Faculty of Science, Mansoura University, Damietta Branch, Egypt

Email

-

City

-

Orcid

-

First Name

Waheba A.

Last Name

Zarouk

MiddleName

-

Affiliation

Molecular genetics and enzymology department, National Research Center,Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Ibrahim E.

Last Name

Mohamed

MiddleName

-

Affiliation

Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

Volume

40

Article Issue

1

Related Issue

3652

Issue Date

2010-07-01

Receive Date

2018-10-23

Publish Date

2010-07-01

Page Start

306

Page End

313

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 40_Issue 1_Pages 306-313.pdf

PDF . 794.4kB

Link

https://ejhm.journals.ekb.eg/article_17380.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=17380

Order

2

Type

Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Identification and Characterization of Nuclear Matrix Protein(s) and assessment of its usefullness for early diagnosis of bladder cancer

Details

Type

Article

Created At

22 Jan 2023