Introduction The present study was carried out to evaluate the effect of aging and anti-aging hormones on the kidney, the thyroid and the testis of aged male albino rats from the physiological and histological points of view.   Material & Methods Thirty five male rats were used in the present study. They were allocated into five groups. The first group (5months old) served as control group and the other remaining groups are (18 months old). The second group 1 ml/kg b.w. corn oil intramuscular injection through a period of two weeks .The third group received 2mg/kg b.w. of melatonin hormone orally daily for two weeks. The fourth group received 0.57 mg/kg b.w. of testosterone hormone via intramuscular injection through two weeks. The fifth group received the same dose of both hormones (Melatonin & Testosterone) for two weeks. Some biochemical parameters of the kidney, the thyroid and histological structure of the testis were examined.   Results The untreated aged group showed insignificant change in urea level with highly significant decrease in creatinine, T₃ and T₄ hormones levels.The melatonin treated group showed significant decrease in urea level with highly significant decrease in creatinine, T₃ and T₄ hormones. The testosterone treated group showed highly significant increase in urea, T₃ and T₄ hormones and highly significant decrease in creatinine level. Whereas, fifth group showed significant decrease in urea accompanied with a highly significant decrease in creatinine and  highly significant increase in T₃ with a significant increase in T₄. The histological changes induced by aging and anti-aging hormones included intertubular haemorrhage, odematous areas present between the seminiferous tubules. The interstitial tissue was degenerated. The degenerated seminiferous tubules revealed maturation arrest in late-stage spermatides.   Conclusion In conclusion, aging and anti-aging hormones administration into adult male rats exerts a clear effect on the kidney and the thyroid functions and on the testicular structure. On the other hand, amelioration in T₃ &T₄ serum level was found in anti-aging treated rats compared with untreated aged rats.