Introduction:   The present study was carried out to evaluate the effect of Sitagliptin (Januvia) on the liver of experimentally induced diabetes in albino rats. Material and Methods: Fifteen adult male albino rats were used and divided into three equal groups. The first group was considered as a control group. In the second group experimental induction of diabetes was performed by intraperitoneal injection of alloxan and left as a diabetic control for three weeks.  The third group was consisted of rats of experimentally induced diabetes and treated by a daily dose of Sitagliptin (Januvia) as oral anti diabetic therapy for three weeks. Two main parameters were performed; the first was microscopic and histochemical studies on the liver tissue while the second was laboratory evaluation of some liver functions. Results: The hepatic tissue was affected by the experimental induction of diabetes in the form of cellular infiltration, hepatic cell cords disarrangement and vascular congestion after three weeks of induction. The fibrous elements as well as mucopolysaccharides contents were greatly reduced. Histochemical changes in the liver enzymes showed mild decrease. Liver function tests showed mild changes. Diabetic changes were gradually returned back to its normal state after the use of daily oral dose of Sitagliptin. Conclusions: The antidiabetic drug (Sitagliptin) could be considered a good therapy in limiting the risk of diabetes Mellitus on liver tissue.