Background: pancreatic carcinoma is one of the leading cancer morbidity and mortality worldwide. Endoscopic Ultrasound (EUS) provides good direct visualization of the pancreas and EUS-FNA raises the accuracy for diagnosing pancreatic malignancies. US-FNA is another established method for diagnosing pancreatic malignancies. Aim: to determine the Role of Percutaneous US-FNA and EUS-FNA in diagnosis of pancreatic lesions. Subjects & Methods: 131 patients with pancreatic masses were included in the study and sub-classified into 2 groups according to the imaging tool used, US-FNA (group I) and EUS-FNA (group II). Results: using the appropriate statistical tools, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and diagnostic accuracy were calculated for both groups. It was 88.2%, 93.9%, 96.8%, 97.5% and 90.1% respectively in group I. It was 77.8%, 100%, 100%, 75% and 86.7% respectively in group II. Conclusion: US-FNA/EUS-FNA are safe reliable tools for diagnosis of pancreatic lesions.