Patients and  methods: The present study consisted of 50 subjects were classified into three groups ; Group (GI) Control group consisted of 10 clinically healthy adult subjects of both sexes free from any liver , kidney or cardiovascular diseases . Group (GII) diabetes mellitus type 1 consisted of 20 patients of both sexes. Group (GIII) diabetes mellitus type 1 with nephropathy consisted of 20 patients of both sexes . All subjects were undergo to the following investigated parameters; Ascorbic acid (vitamin C) ,Catalase ,Total antioxidant capacity, Aldolase and  Pyruvate kinase enzyme.                                                          Results: vitamins C, catalase, total antioxidant capacity enzymes were highly significant decreased (P < 0. 01) in  diabetes mellitus type 1 (GII)  and diabetes mellitus type 1 with  nephropathy (GIII)  when compared to the control group. Adolase activity washighly significant increased (P < 0. 01) in diabetes mellitus type 1 with  nephropathy (GIII) when compared to the control group . Pyruvate Kinase activitywashighly significant increased (P < 0. 01) in diabetes mellitus type 1 (GII) when compared to the control group . Conclusion: The antioxidant  and enzymes can be used for follow up in patients  suffering from diabetes mellitus type 1   and predict other complications.