Aim of the work: Investigation of the histological and histochemical changes in the lung of male albino rats exposed to mobile phone radiation and the possible protective role of vitamin E or Silymarin. Material and Methods: The present study was carried out on 36 adult male albino rats (Sprague dawly); they were divided equally into 6 groups (C group: control rats; R group: rats exposed to 900 MHz (2h /day) of radiofrequency electromagnetic field (RF-EMF) radiation; S group: positive control rats given Silymarin; E group: positive control rats given Vitamin E; R+S group: rats treated with Silymarin post EMF irradiation and R+E group treated with vitamin E post EMF irradiation). Results: Rats exposed to mobile phone radiation showed numerous histological and histochemical changes; these changes were ameliorated by using vitamin E or Silymarin. Vitamin E showed anti-damaging effect of lung tissue exposed to mobile phone radiation more than Silymarin. Conclusion: The present study showed that the natural anti-oxidant Silymarin and vitamin E could protect the lung tissue from the damage produced after the exposure to mobile phone radiation. Vitamin E showed significant anti-damaging effect more than the anti-damaging effect of Silymarin.