Aim of the work-The present work aimed to investigate the ability of Hesperidine (HDN) as an antioxidant to retard development of renal toxicity induced by CCL4 in rat. Hesperidin (HDN), a citrus bioflavonoid, decreases the oxidative stress produced by carbon tetrachloride in rat kidney. Exposure to CCl4 induces acute and chronic renal injuries. The present study was designed to evaluate the protective effect of hesperidin, on rat kidney damaged by CCl4. Material and methods- Animals were divided into five groups pretreated with hesperidin (HDN) (100 and 200 mg/kg orally) for 10 days and then challenged with CCl4 (2 ml/kg/s.c.) of in olive oil subcutaneously. Rats were sacrificed by carotid bleeding under ether anesthesia. Results: The present results showed that the antioxidant properties of hesperidine might be the main factor responsible for its strong protective action on CCl4-induced nephrotoxicity. Conclusion- This study proved that hesperidin has a protective effect on the renal tissue of rat and the degree of improvement varies in intensity according the dose of Hesperidin