Blackgrond:plant lectins, carbohydrate binding proteins, are distributed in many species of medicinal plants mainly those belong to legumonase family. This study aimed to investigate the anticancer activity of the lectin extracted from Pisum sativum on Human Hepatocellular carcinoma (HepG2). Materials and Methods:the morphological signs of apoptosis were examined and the ability of lectin to induce alteration in both pro-apoptotic and anti-apoptotic such as Bax, IκBα, P53 and Bcl2 genes were analyzed using RT-QPCR method. The safety usage pattern of P. sativum lectin was evaluated on human HepG2 cell lines. Results: lectin displayed high antioxidant activity on the Hep2 cells when compared with 5FU (anti-cancer drug as a control). Lectin dilution that exhibits LC50 on HepG2 cells was found to touch 25% from the original concentration (100%).  The extracted lectin was found with inhibition percentage in cellular viability touching 80.5% with 67.6% inhibits the integration of BrdU in the HepG2 proliferated cells. HepG2 treated cells showed apparent nuclear condensation after 16 h of treatment as lectin had the ability to upregulate the gene expression of P53 and IκBα and down regulates Bax and Bcl2. Conclusion: lectin may have a vigorous role in achievement of hepatocarcinoma therapy plan.