Background: shrimps are extremely good source of high nutritive value making them a very healthy choice for human food.   Aim of the work: to examine the changes induced by seasonal variation in Peanuts semisulcatusbiochemical composition and nutritive value.    The present work aimed also to screening of crude shell extract for bioactivity and economic importance.  Materials and methods:   The proximate composition and biochemical constituents were analyzed seasonally in the muscle tissue of Penaeus semisulcatus. The Crude protein, lipid, carbohydrate, calorific value, moisture and ash were quantified in the Penaeid Prawn. Extraction, isolation, and identification of some bioactive marine natural products were done for study its effect on bacteria, fungi and Larynx cancer. Results:  there is seasonal variation of the nutritional status of edible Portion of prawn. Statistical analysis for the measured results of the correlation values for each of measurements with the seasons of the year. The correlation was a positive weak. The results of Antimicrobial activity of Penaeus semisulcatus shell extract indicated a significant effect differences between the groups treated with shell extract (1.5, 2.5, and 5 mg/mL) compared to the con­trol group.  Also, evaluation of viability of Hep -2 cell line post treatment with shell extract ofPenaeus semisulcatus using Sufranin uptake assay as (M T T) uptake and Microscopic examination indicated a significant difference between the groups treated with shell extract compared to the con­trol group.  Cell viability decreased depending on dose or concentration.  The shell extract inhibited the proliferation of a Larynx cancer cell line in a dose dependent manner. Conclusion: the results of the present study reported that, the nutritional values of shrimp muscle constituents were different from season to the other, with positive weak correlation.  Also, Shell extract can be used as antibacterial, antifungal and anticancer.