Aim of the work: this study aimed to detect assessment of injection practice using WHO safe injection tool then implantation of education program and post intervention evaluation. Methods: this study was anon randomized intervention study performed in Zagazig University Hospitals and targeting nurses , 700 observation before and 700 observation after intervention starting by evaluation of injection practice using WHO safe injection tool then training on safe injection practice applied for nurse staff then post training evaluation of practice. Results: hand hygiene soap and alcohol rub was available in hospitals sitting in 80%,85% of observation and after intervention increased up to 95% and98%  with a significant difference. Safety boxes were available for sharp disposal before intervention and become 100% available after intervention. Hospital supply 100% percent single use syringes for each patient, but the problem was for drug administration process due to deficient in cleaning of drug preparation area 88% of pre intervention observation, but practice improved to 98% after intervention with a significant difference; sharp disposal practice was improved after intervention up to 98% with significant difference. 67% of observation used non touch technique and changed to 95% after intervention with high significant difference   .Vaccination coverage reached 100% after intervention and percent of nurses who received training on safe injection was 45% increased to 100% after intervention, also post needle stick injury counseling was 57% reached 100%after intervention. Conclusions: training and education of nursing staff on safe injection practice was crucial for improvement of safe injection practice and improved both staff and patient safety.