Background:  The most common risk factors for gallstones formations are old age, female gender, obesity and diabetes mellitus. However, information about the prevalence and significant risk factors in Kingdom of Saudi Arabia (KSA) are limited. Objectives: Evaluating the prevalence and risk factors of gallstone disease among Saudi population in Riyadh, Kingdom of Saudi Arabia (KSA). Methods: A cross sectional study that included 500 subjects adult Saudi population who were randomly chosen from February –June 2017. The volunteers were subjected to clinical evaluation, laboratory investigations and ultrasound examination. Results: The overall prevalence of gallstone disease was 8.6%. The older age, female gender, physical activities and obesity are key factors in the gallstones progression. Also, diabetes, liver enzymes and the lipid profile were modifiable risk factors for gallstones. Conclusion: The risk factors for gallstones formation are old age, female gender, physical activities and obesity were key factors in the gallstones progression.