Objectives: The purpose of the study was to measure and compare mean enamel loss caused by microabrasion treatment of different abrasive materials under a fixed variable. Materials and methods: In this laboratory study, three commonly used abrasive materials were compared: Dental Pumice, Zircate® Prophy Paste, Opalustre® and a controlled group (distilled water). Hundred extracted human premolars were randomly allocated into four groups; each group was subjected to fixed pressure, time and rotational speed mimicking dental office setting. Results: Data was collected and statistically analyzed.  The results of the study (p < 0.01) was statistically significant and showed that Opalustre® caused the highest mean difference in enamel loss. Followed by dental Pumice and Prophy paste in order with minimal difference between them. And the controlled group (distilled water) had the least mean enamel loss. Conclusion: microabrasion offers a great approach in treating enamel defect/staining in a minimally invasive procedure in order to achieve acceptable aesthetic results while preserving the tooth's microstructure as much as possible. As shown in the result; Combined chemical acid with mechanical abrasive particles produce most effective results.