ABSTRACT Cystic fibrosis (CF) is a disease characterised by the depletion of Airway Surface Liquid (ASL) height. Lipoxin A₄ was found to be effective in restoring ASL back to normal by acting on ENaC. The signalling mechanisms involved in lipoxin effect were investigated by testing the action of a calcium chelator (Bapta-AM) and Protein kinase C inhibitor (GF 109203X) on NuLi-1 and CuFi-1 cells. Drug's efficacy was monitored by conducting Ussing chamber experiments and confocal microscopy. Results showed that calcium has a major contribution to the lipoxin A₄ effects while PKC has a limited role.  However, our data might lack accuracy due to shortage of time to conduct more experiments.