Background: Glucose-6-phosphate dehydrogenase deficiency is an X linked recessive disorder expressed mostly in males. It is the most common enzyme deficiency worldwide, causes a spectrum of disease including neonatal hyperbilirubinemia, acute hemolysis, and chronic hemolysis. The aim of the study was to determine prevalence, risk factors of G6FD deficiency and its symptoms among general population of Dammam, Eastern Province of Saudi Arabia. Methods: A cross-sectional study was conducted during the period from 1 March 2016 to 30 July 2017. Data was collected from 182 individuals. Systematic random sampling technique was followed. Data was collected by using predesigned online questionnaire. Results: The overall prevalence of favism was 17%, of them, 67.7% were females and 32.3% were males. 15.2% of cases and 26.9% of the total sample had family history of the disease. Among the studied cases, 48.4% complained of pallor, 45.2% headache, 45.2% drowsiness, 12.9% nausea, 32.3% back pain, 64.5% tiredness, 25.8% abdominal pain, 38.7% discoloration of urine, 29% foot pain, 16.1% low grade fever and 19.4% complained of jaundice. Regarding risk factors of favism; 32.3% reported excess beans intake, 12.9% antibiotic intake and only 9.7% reported bacterial or viral infection. Conclusions: Glucose-6-Phosphate Dehydrogenase deficiency is a common disorder as it affects 17% of the study population of Dammam, Eastern Province of Saudi Arabia. It was more common in females than males. Health education sessions and further epidemiological studies are required because early detection and prevention is the key strategy for successful management and control of this genetic disease.