Seafood in general, shrimps and prawns in particular, are highly nutritious with good source of protein and amino acids^. The present study was conducted to evaluate the nutrient value in males and females of green tiger prawn, P. semisulcatus. Twenty specimen were collected from land fisheries in Mediterranean Sea (Alexandria) and from markets (India). The results showed that the highest values of lipid contents were measured in the female and male edible muscles (P. semisulcatus at Mediterranean Sea (Alexandria) compared to female and male edible muscles of the same species from market (India). The carbohydrate contents in the male edible muscles of P. semisulcatus were higher compared to females in the studied regions. Sixteen amino acids have been determined in edible muscle of P. semisulcatus, among these, nine essential amino acids (EAAs) and seven non- essential amino acids (NEAAs) were estimated in both sexes from two regions. The quantities of amino acids vary considerably between sexes. The fatty acid contents from the muscles of the P. semisulcatus showed the presence of fifteen individual fatty acids, which include seven saturated fatty, three mono and five polyunsaturated fatty acids (MUFA and PUFA). Twelve protein bands were detected in males of P. semisulcatus (12 bands); while the females had 13 bands from market (India) compared to 9 bands of both males and females from Mediterranean Sea (Alexandria). Conclusion: The present study clearly indicated that the nutritional value of P. semisulcatus is very well as food and health care.