Background: Influenza is an airborne communicable disease; it is effectively can be prevented by vaccination. Healthcare workers (HCWs) contact with different patients with different diseases and different immunity levels, vaccination of healthcare workers for influenza can protect both healthcare workers and their patients. Although the vaccine is available and proved to be safe, there is low rate in uptake of vaccination. Aim: To estimate the influenza vaccination rate among HCWs and determine the role of different factors on acceptance of influenza vaccine. Methods: This cross-sectional study was conducted on all healthcare workers at Al-Wazarat health care center, PSMMC, Riyadh, Saudi Arabia. A well-established questionnaire was used in this study to obtain required data including demographics and vaccination state of participants and their beliefs about the vaccination. Results: There were 50.5% of HCWs have received influenza vaccination for 2016-2017 season. Several factors were associated with vaccination state including age (P-value=0.04), nationality (P-value=0.01) and having chronic disease (P-value=0.004). The mean score for HCWs‘attitude was 37.6±5.67, and it significantly increased in vaccinated (39.15±5.23) than non-vaccinated participants (36.19±5.7). Nature of work and nationality significantly affected the mean score (P-value=0.02, 0.01) respectively. Conclusion: Vaccination uptake rate was low among health care workers with moderate acceptance rate for vaccination, vaccinated persons had higher acceptance score than non-vaccinated one.