Background: health status and lifestyle pattern are examples of health-related practice and the determinant of the individual's future health, performance and life expectancy. World health organization (WHO) indicated that 60% of an individual's health-related quality of life depends on life style. The health status can be controlled by adopting the important aspects for a healthy life style, dietary habit, stress management, physical activity and smoking cessation. Methods: this cross-sectional study was conducted in College of Medicine, Qassim University, Saudi Arabia to assess the health promoting lifestyle and associated factors of medical students. Data was collected using the standardized HPLP II Questionnaires. The questionnaires were covered in two parts. The first part includes demographic questions and second part included 52 questions related to Health- Promoting Lifestyle Profile (HPLP-II).426 out of 652 students were participated with response rate of 65%. Results: this study showed that the average of total score regarding to health promotion for all participants related to the different variable is too less 2.33 out of 4. In addition, there is no statistical significant relation between HPLP Total Scoreand the different study variables; P Value > 0.05. Our study indicated there was strong positive correlation between HPLP total score and subscales level of significant 0.05 and 0.01.