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Adenovirus Association with Graft versus Host Disease in Paediatric Bone Marrow Transplantation Recipients

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Last updated: 24 Dec 2024

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Abstract

Background: Adenovirus (Ad) infections have delayed clearance in paediatric patients. The immune suppression that occurs after hematopoietic stem cell transplantation (HSCT) can reactivate adenovirus, resulting in life- to assess adenovirus infection in recipients of bone marrow transplantation and to find out if there is an association between adenovirus infection and the occurrence of graft versus host disease (GVHD) in these patients. Patients and methods: The study was conducted on 30 pediatric patients admitted to Nasser institute, for bone marrow transplantation. Serum and stool samples were collected one day prior to bone marrow transplantation, and every one or two weeks afterwards till the patient completed 100 days after HSCT. The adenoviral DNA was monitored in the patient‟s serum and stool samples by quantitative real time polymerase chain reaction (qPCR). The stool samples that tested positive for the presence of adenovirus were processed by cell culture technique for isolation of the virus. Results: Adenoviral DNA was detected in the fecal samples of 11 out of 30 patients (36.6% of cases); the viral load ranged from 1.2 X 103 copies/gram, to 8.4 X 107 copies per gram, two patients had 2 positive stool samples with rising titer indicating the reactivation of the adenovirus. The adenoviral DNA was detected in the serum samples of only two patients (6.6% of cases), with a low titer, one of them was only 500 copies/ml and the other one was 1.6 X 104 copies /ml. The remaining serum samples of the patients who shed the adenovirus in their stool were all negative for adenoviral DNA. These two patients, who had Ad DNA in their serum, had no adenoviral shedding in their stool. The adenovirus reactivation was not associated with increased risk of developing GVHD, diarrhea or CMV reactivation.
Conclusion: although the adenovirus was shed in the stool of 9 patients, the viral shedding was not associated with increased risk of developing GVHD, diarrhea or CMV reactivation.

Keywords

Adenovirus, hematopoietic stem cell transplantation (HSCT), graft versus host disease (GVHD), Bone Marrow Transplantation

Authors

First Name

Arwa Kamhawy 1, Kouka Saad Eldin Abd Elwahab 1,

Last Name

Jaylan Ayoub 1,

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Affiliation

1-Microbiology and Immunology Department, Faculty of Medicine for Girls, Al Azhar University, Cairo

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Orcid

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First Name

Abd Elrahman Zekri 2,

Last Name

Alaa Elhaddad 3

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Affiliation

2-Molecular Biology and Virology Department, National Cancer Institute, Cairo University, Cairo, 3- Paediatric Oncology Department, National Cancer Institute, Cairo University, Cairo, Egypt

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Volume

71

Article Issue

5

Related Issue

1649

Issue Date

2018-04-01

Receive Date

2018-02-23

Publish Date

2018-04-01

Page Start

3,086

Page End

3,092

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 71_Issue 5_Pages 3086-3092.pdf

PDF . 411.5kB

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https://ejhm.journals.ekb.eg/article_8580.html

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https://ejhm.journals.ekb.eg/service?article_code=8580

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Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Adenovirus Association with Graft versus Host Disease in Paediatric Bone Marrow Transplantation Recipients

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Article

Created At

22 Jan 2023