Objectives: To assess the Knowledge, attitude, and practice of Doctors and Medical Students towards Stem Cell use in the Management of Diabetes Mellitus. Methods: This cross-sectional descriptive study was conducted to assess the knowledge, attitude and practice of stem cell therapy among 120 Doctors and medical students in Tabuk City, Saudi Arabia during the period from April to December 2017, participants signed a written informed consent, then responded to a self-reported twenty-six item questionnaire. (Ten questions to assess knowledge, 6 questions to assess practice, and 10 questions to assess attitude). The data were analyzed by The Statistical Package for Social Sciences (SPSS), and the independent-samples t-test was used to compare men and women, doctors and students. A P-value of <0.05 was considered significant. Results: Out of 120 doctors (n=75) and medical students (n=45), the male dominance was evident (81.6%). The knowledge was poor in 21%, fair in 76.5%, good in 2.5%, while no one scored excellently. The attitude score was excellent in only 0.8%, good in 9.2%, fair in 73.1% and poor in 16.8%. Regarding practice, excellent, good, fair, and poor were reported in 14.3%, 52.9%, 21.8%, and 10.9% respectively. No significant statistical gender differences were evident between doctors, and medical students. Conclusion: The knowledge, attitude, and practice were suboptimal among doctors and medical students in Tabuk, Saudi Arabia. An educational intervention to upgrade the knowledge is highly needed.