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277422

Molecular Characterization of Bovine Rotaviruses and Coronaviruses in Diarrheic Calves in Egypt (2014-2019)

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Clinical Science, Surgery and Veterinary Diseases (Pathology, Clinical…ltry Diseases, Fish Diseases and Management, Surgery, Theriogenology)

Abstract

Bovine Rotaviruses group A (BRVA) and Bovine Coronaviruses (BCoV) are the most prevalent viral agent worldwide in diarrheic calves aged less than 6 weeks, causing economic losses due to retarded growth, increased susceptibility to other infections, treatment cost, and calf mortalities. This study aimed to detect and molecularly characterize BRVA and BCoV from diarrhetic calves. A total of 82 fecal samples were collected from calves aged less than one month from three Egyptian governorates (Alexandria, Ismailia, and Sharqia). All fecal samples were tested for BRVA and BCoV by using probe based quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Consequently, positive samples contain relatively high viral genomic load were examined by RT-PCR for amplification of viral protein 7 (VP7) and viral protein 4 (VP4) genes (G- and P- typing) for BRV and full length S1 gene for BCoV. Out of 82 of tested samples, 14 (17.1%) and 22 (26.8%) were positive by qRT-PCR for BRV and BCoV, respectively. Only three and five samples had relatively high genomic load for BRV and BCoV, respectively for further testing by RT-PCR. BRV G-type was found in two samples and P-type was detected in one sample. The sequence analysis and phylogenetic tree typed these positive samples as P11 and G10. The sequences and phylogenetic analysis of BCoV positive strains (n=5) showed closely related viruses to each other and similar to previously characterized strains in Egypt since 2014. Further studies are required to antigenically characterize the circulating BRV and BCoV in Egypt.

DOI

10.21608/zvjz.2022.179675.1197

Keywords

Rotaviruses, Coronaviruses, P typing, P11, S1 segment, Egypt

Authors

First Name

Esraa

Last Name

Elsadek

MiddleName

-

Affiliation

Animal Health Research Institute

Email

dr.esraa_psg@vet.suez.edu.eg

City

Ismailia

Orcid

-

First Name

Mohamed

Last Name

Elshehidy

MiddleName

-

Affiliation

department of virology, Faculty of veterinary medicine , suez canal university

Email

el_shahidy@yahoo.com

City

ismailia

Orcid

-

First Name

Momtaz

Last Name

Shahein

MiddleName

-

Affiliation

animal health research institute

Email

momtaz.shahein@yahoo.com

City

cairo

Orcid

-

First Name

Mohamed

Last Name

Maarof

MiddleName

-

Affiliation

department of virology, faculty of veterinary medicine, suez canal university

Email

mohamed_maarof@hotmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

soltan

MiddleName

-

Affiliation

department of veterinary medicine, infectious diseases

Email

msoltan@vet.suez.edu.eg

City

ismailia

Orcid

-

Volume

50

Article Issue

4

Related Issue

38547

Issue Date

2022-12-01

Receive Date

2022-12-15

Publish Date

2022-12-01

Page Start

320

Page End

334

Print ISSN

1110-1458

Online ISSN

2357-075X

Link

https://zvjz.journals.ekb.eg/article_277422.html

Detail API

https://zvjz.journals.ekb.eg/service?article_code=277422

Order

277,422

Type

Original Article

Type Code

601

Publication Type

Journal

Publication Title

Zagazig Veterinary Journal

Publication Link

https://zvjz.journals.ekb.eg/

MainTitle

Molecular Characterization of Bovine Rotaviruses and Coronaviruses in Diarrheic Calves in Egypt (2014-2019)

Details

Type

Article

Created At

22 Jan 2023