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116295

Effect of Zingiber officinale and Nigella sativa on Streptozotocin-Diabetic Rats with Reference to Biochemical and Hematological Studies

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Academic and Pre-clinical Veterinary Sciences (Physiology, Histology, …rology, Parasitology, Pharmacology, Forensic Medicine and Toxicology)

Abstract

This study was aimed to assess the potential ameliorative effect of the ethanolic extract of Zingiber officinale (Z. officinale; ginger) and Nigella sativa (N. sativa; black seed) on streptozotocin (STZ)-diabetic rats.  Fifty albino rats of Wistar strain were randomly divided into five equal groups. Group 1 received saline orally (a control group). Groups 2 -5 were experimentally exposed to diabetes via intraperitoneal (i.p.) injection of STZ (55 mg/kg BW.). The 2nd  group was left without treatment as diabetic non-treated control, while the 3rd  to 5th  groups were orally treated with metformin (100mg/kg BW), ethanolic extract of ginger (200mg/kg BW.) and N. sativa (300mg/kg BW.), respectively for 21 consecutive days.Blood and pancreatic tissue samples were collected at the end of the experiment for biochemical analysis. The results revealed that blood glucose, fructosamine and insulin levels were improved following treatment by metformin (99.00±4.76, 198.69±5.25 and29.82±2.35, respectively),and ethanol extract of ginger (105.20±4.87, 317.48±17.79 and13.64±0.72, respectively). Meanwhile, N. sativa extract produced insignificant decrease in glucose level (201.60±14.91) and significant improvement in serum fructosamine and insulin levels (273.20±6.39 and 21.65±1.78) if matched with the diabetic control group on the 3rd week of the experiment. Treating of diabetic rats with metformin and N. sativa extract elicited a significant increase in blood RBCs count (6.21±0.170 and 5.63±0.135), Hb concentration (12.96±0.229 and 12.00±0.141), PCV% (46.40±1.077 and 74.72±1.085),  tissue catalase (0.32 ±0.012 and 0.25±0 .009) and superoxide dismutase activities (19.44±2.16 and 12.77±0.914), respectivelywhen compared with the diabetic group. However, administration with ginger extract produced variable changes in the erythrogram and leukogram. This study provided that Z. officinale and N. sativa extracts have significant effects on some biochemical and hematological assays, as compared with metformin known as standard antidiabetic drug. Consequently, the herbal extracts under current inquiry could have a role in alleviating the risk of some chronic complications of diabetes.

DOI

10.21608/zvjz.2020.29101.1108

Keywords

Key words: metformin, Ginger, Nigella Sativa, nicotinamide, diabetes

Authors

First Name

Hosny

Last Name

Ibrahim

MiddleName

-

Affiliation

Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt

Email

dr.hibrahim951@yahoo.com

City

Sharkia

Orcid

-

First Name

Mohamed

Last Name

Hashem

MiddleName

-

Affiliation

Clinical Pathology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt

Email

mhashem.vet@gmail.com

City

Sharkia

Orcid

-

First Name

Nagah

Last Name

Mohamed

MiddleName

-

Affiliation

Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt

Email

edreesnagah@gmail.com

City

-

Orcid

-

First Name

Aliaa

Last Name

Abd El-Rahman

MiddleName

-

Affiliation

Veterinary Directorate, Zagazig, Sharkia Province, Egypt

Email

aliaazeitoun84@gmail.com

City

zagazig

Orcid

-

Volume

48

Article Issue

3

Related Issue

17569

Issue Date

2020-09-01

Receive Date

2020-06-04

Publish Date

2020-09-01

Page Start

306

Page End

318

Print ISSN

1110-1458

Online ISSN

2357-075X

Link

https://zvjz.journals.ekb.eg/article_116295.html

Detail API

https://zvjz.journals.ekb.eg/service?article_code=116295

Order

8

Type

Original Article

Type Code

601

Publication Type

Journal

Publication Title

Zagazig Veterinary Journal

Publication Link

https://zvjz.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023