Publicity of pumpkins in traditional medicine for relieving diverse disturbances such as hypertension, arthritis, hyperglycemia, inflammation, dyslipidemia, fungal or bacterial infections, tumors, and intestinal parasites made a highlight for more research on pumpkin fruits as well as seeds. Multiple studies have been fulfilled on animal models, clinical trials or cell cultures to assess these medicinal activities. Pumpkin is admitted for its ingested seeds, fruits, and greens. Pumpkin seeds are enhanced by micro- and macro-constituent compositions include proteins, antioxidative phenolic compounds, tocopherols, triterpenes, saponins, phytosterols, lignans, and carotenoids; among these compounds: D-chiro-inositol, trigonelline, and nicotinic acid, those are reflected as insulin action mediators. Pumpkin seeds are also fortified with polyunsaturated fatty acids (PUFA), fibers, vitamins, and minerals including zinc, iron, magnesium, calcium, manganese, and copper, thus pumpkin seeds have become commonly used as health booster or herbal therapy for human as well as animals, and the food technologists have conquered the commercial food and alternative medicine sectors with pumpkin products. Furthermore, Food companies intend to exploit pumpkin seeds in the starters and snacks industry. This review article provides an update on the therapeutic impacts of pumpkin seeds and mechanisms by which they may limit the risk of diseases.