Aim: The aim of this study was to investigate whether subcutaneous (S/C) or intramuscular (I/M) is better in vaccination against foot-and mouth disease (FMD), in addition, to determination of the most protective dose concerning the economic point of view.
Forty  animals were divided into ten groups ,each group eight of sheep were vaccinated with different doses of the bivalent oil FMD vaccine (with 1 ml, 0.5 ml, 0.25 ml and 0.125 ml doses) either S/C or I/M. The experiment included challenged non-vaccinated control group and non-challenged-non-vaccinated control groups. Four weeks post vaccination all animal groups were challenged by inoculation intradermolingual (IDL) with 10.000 ID₅₀ (infective dose fifty) sheep adapted challenge FMD (O & A types) virus and subjected to clinical observation for 8 days. The results showed that the vaccinated animal with a full, ½, ¼ dose injected either S/C or I/M were able to withstand the virus infection while animals vaccinated with ¹/₈ dose didn't