Out of 100 fecal samples from Cattle, 24%gave positive results to Giardiasis, and also out of 100 stool samples from man occupationally contacts with cattle in different age and seasons, 32% were positives. The percentage of human Giardiasis among man in different ages (infants, preschool children and adolescents) in Kaloubia Province revealed that 11% 10%, and 8%  respectively and lastly in adults 3% while the percentage of human Giardiasis among man in different seasons revealed that 12% , 10 and 8% during the summer season , autumn, and spring respectively and the lowest infectious rate was in winter 2% . In this assay by using heminested PCR-restriction fragment length polymorphism analysis for the detection and genotyping of Giardia lamblia on the basis of polymorphism in the triose phosphate isomerase (tpi) gene. According the taxonomy of Giardia, genotype indicate that the assemblages A and B classified as zoonotic type and also represented result may give  us indication  to the possibility of zoonotic potential of  giardiasis.