In this study, a trivalent autogenous inactivated P.multocida vaccine was prepared. The prepared vaccine contained three different P. multocida strains which were previously isolated from different localities at Sharkia Governorate and identified by biochemical and molecular methods. The protective effect of the prepared vaccine was evaluated through estimation of the humeral immune response using indirect heamagglutination test (IHA); challenge infection and field evaluation. Pasteurella multocida strains were inactivated with formalin (0.5%) and adjuvanted with montanide oil (ISA -70). Repeated immunization raised up the specific antibody level eight times in vaccinated rabbits. It conferred protection against challenge with homologous strains in commercial rabbits. In the field study a positive effect of vaccination on nasal colonization with P. multocida was found.