Brucellosis is an important disease of public health significance that leads to severe losses. The current study was carried out to identify the recovered Brucella species , and its prevalence in cows and ewes in Dakahlia and Damietta Governorates during May 2018 to March 2019, with the hemato-biochemical analyses in the positive cases. In the current study, B. melitensis biovar 3 was identified with a total prevalence of 8.75% and 6.98% in cows and ewes, respectively. Hematological results denoted a normocytic normochromic anemia in infected cows and ewes at the 2^nd and 4^th weeks after abortion group (GP) 2 and GP.3, respectively, with leucocytosis especially in GP.3. Significant hypoproteinemia and hypoalbuminemia were detected in infected cows at   the 2^nd week after abortion, with non-significant changes at 4^th week after abortion. While Brucellosis-infected ewes showed a significant (P < 0.05) hypoproteinemia and hypoalbuminemia at both 2^nd and 4^th weeks post abortion. The serum AST, ALT, ALP and LDH activities and levels of IL-6 and TNF-α were markedly elevated (P < 0.05) in both infected cow and ewes with markedly lowered serum urea level. Whereas serum glucose level was significantly decreased (P < 0.05) in infected cows (GPs 2 and 3), as well as, infected ewes of GP. 2, with non-significant changes in infected ewes of GP3. The serum creatinine level showed non-significant changes in all infected cows and ewes. Serum immunoglobulin M was significantly increased in both infected cows and ewes of GPs 2 and 3, while immunoglobulin G was significantly elevated in both infected cow and ewes of GP3. Moreover, phagocytic percent and index showed a significant decrease in both infected cows and ewes (GPs2 and 3). Thus, evaluations of hematological, biochemical and immunological parameters together with specific diagnostic tests are necessary to obtain more reliable results for the diagnosis of Brucellosis in cows and ewes.