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66826

Coliforms Contamination in Raw Milk and Some Dairy Products with Special Reference to Comparative Identification of Enterobacter spp.

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Animal Health, Nutrition and Food Control (Veterinary Public Health, Animal Wealth Development, Animal Nutrition, Zoonoses, Food Control)

Abstract

The current study was undertaken to examine 200 sample of raw milk and some dairy products (Kariesh cheese, plain yoghurt, milk powder and infant formula) for contamination with Coliform group especially Enterobacter spp. Coliforms were detected in; 42/50 (84%) raw milk samples from farmers' houses, 25/30 (83.33%) kariesh cheese samples and 23/30 (76.67%) plain yoghurt samples, however, they could not be detected in any of raw milk samples from dairy shops, milk powder and infant formula samples. The mean values of coliforms in the examined samples were 2.80x106 ±0.73x105, 2.30×106 ±0.75×104 and 1.08×106 ± 1.50×104 cfu /ml or gm in raw milk from farmers' houses, kariesh cheese and plain yoghurt samples, respectively. The biochemically identified coliforms were E. aerogenes, E. agglomerans, E. cloacae, C. diversus, C. freundii, E.coli, K. oxytoca and K. pneumonia with respective percentages of; 1.19, 1.19, 2.38, 25, 15.48, 26.19, 13.09 and 15.48, in raw milk from farmers' houses, 6, 0, 2, 28, 10, 22, 0, and 32 in kariesh cheese, 0, 0, 2.12, 23.91, 0, 36.96, 26.09, 10.87 in plain yoghurt. Comparative identification of isolated Enterobacter spp.by standard biochemical methods and Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry revealed that the total conformity of identification of Enterobacter strains between standard biochemical technique and MALDI-TOF MS technique was 66.6%, where, it ranged from 50% to 100 for E. aerogenes and E. cloaca, respectively. However, the only identified E. agglomerans isolatesfrom raw milk could not be confirmed by MALDI-TOF MS technique. It has been shown through these results that the presence of coliform and Enterobacter bacteria is an evidence of the lack of health requirements and thermal treatments of raw milk and some of its products (kariesh cheese and yoghurt).

DOI

10.21608/zvjz.2019.14730.1059

Keywords

Milk, Dairy products, coliforms, Enterobacter, MALDI-TOF MS

Authors

First Name

Sally

Last Name

Fathi

MiddleName

S.

Affiliation

Directorate of Veterinary Medicine, Mansoura - General Organization for Veterinary Services, 35511, Egypt

Email

sallysoliman505@gmail.com

City

mansoura

Orcid

-

First Name

Asmaa

Last Name

Mohamed

MiddleName

S.

Affiliation

Food Control Department, Faculty of Veterinary Medicine, Zagazig Univeristy, 44511, Zagazig Egypt

Email

drasmaasalah@yahoo.com

City

-

Orcid

-

First Name

Magdy

Last Name

El- Sayed

MiddleName

S.H.

Affiliation

Food Control Department, Faculty of Veterinary Medicine, Zagazig Univeristy, 44511, Zagazig Egypt

Email

magdyelnagar62@gmail.com

City

-

Orcid

-

Volume

47

Article Issue

4

Related Issue

10094

Issue Date

2019-12-01

Receive Date

2019-07-11

Publish Date

2019-12-01

Page Start

388

Page End

397

Print ISSN

1110-1458

Online ISSN

2357-075X

Link

https://zvjz.journals.ekb.eg/article_66826.html

Detail API

https://zvjz.journals.ekb.eg/service?article_code=66826

Order

5

Type

Original Article

Type Code

601

Publication Type

Journal

Publication Title

Zagazig Veterinary Journal

Publication Link

https://zvjz.journals.ekb.eg/

MainTitle

Coliforms Contamination in Raw Milk and Some Dairy Products with Special Reference to Comparative Identification of Enterobacter spp.

Details

Type

Article

Created At

22 Jan 2023