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60195

Quantification of Antibodies in Sera of Vaccinated Chickens against Infectious Bronchitis Virus by Haemagglutination Inhibition Test

Article

Last updated: 03 Jan 2025

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-

Tags

Academic and Pre-clinical Veterinary Sciences (Physiology, Histology, …rology, Parasitology, Pharmacology, Forensic Medicine and Toxicology)

Abstract

A trypsin-induced haemagglutination inhibition (THI) assay was standardized to achieve a quantification of haemagglutinating-inhibiting antibodies against infectious bronchitis virus (IBV) in the sera of chickens vaccinated with different types of commercial imported IBV vaccines. A total of forty four IBV vaccines representing as twenty two live vaccines containing either classical or variant strains and twenty two inactivated vaccines were examined among (THI) assay using trypsin treated classical IB antigen. In a trial for studying the correlation between the results of THI and the other serological tests (ELISA and serum neutralization test "SNT"). The former tests were performed on the same serum samples used in (THI) test. The results showed that the calculated mean HI titers for different examined serum samples ranged between 7.7 log2 to 7.9 log2, against live IB variants vaccines (CR88 and 4/91 respectively), 8.7 log2 to 9.2 log2 against classical type (Ma5, H120) vaccines, 9.1 log2 (H120+D274) and 6.8 log2 against inactivated vaccines. It was observed that quantification of antibodies in sera of chickens against infectious bronchitis virus by HI test had correlation with those obtained by either ELISA or SNT tests.

DOI

10.21608/zvjz.2014.60195

Authors

First Name

Soliman

Last Name

IMA

MiddleName

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Affiliation

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Orcid

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First Name

Ekram

Last Name

Salama

MiddleName

-

Affiliation

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Email

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Orcid

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First Name

Nehal

Last Name

S Abdel Rahman

MiddleName

-

Affiliation

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Email

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City

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Orcid

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First Name

Eman

Last Name

M Ebied

MiddleName

-

Affiliation

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Email

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City

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Orcid

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First Name

Hanan

Last Name

A Ahmed

MiddleName

-

Affiliation

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Email

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Orcid

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First Name

Susan

Last Name

S El-Mahdy

MiddleName

-

Affiliation

-

Email

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City

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Orcid

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Volume

42

Article Issue

3

Related Issue

9037

Issue Date

2014-09-01

Receive Date

2019-11-19

Publish Date

2014-09-01

Page Start

217

Page End

224

Print ISSN

1110-1458

Online ISSN

2357-075X

Link

https://zvjz.journals.ekb.eg/article_60195.html

Detail API

https://zvjz.journals.ekb.eg/service?article_code=60195

Order

21

Type

Original Article

Type Code

601

Publication Type

Journal

Publication Title

Zagazig Veterinary Journal

Publication Link

https://zvjz.journals.ekb.eg/

MainTitle

Quantification of Antibodies in Sera of Vaccinated Chickens against Infectious Bronchitis Virus by Haemagglutination Inhibition Test

Details

Type

Article

Created At

22 Jan 2023