The objective of this research was to describetheanatomical and histological structure of the prostate gland in buffalo bulls. This study was carried out on the prostate gland of 25 animals. The gland consisted of two parts; Corpus prostatae and Pars disseminate prostatae. The shape of the former part was variable (band like, two lobed connected by an isthmus (Isthmus prostatae) and three lobed gland). It was situated transversally on the dorsal aspect of the pelvic urethra just caudal to the dorsal fibrous cord of the vesicular gland. Pars disseminata prostatae occupied in the entire length of the pelvic urethra. Microscopically, the capsuleof the gland was fiberomuscular in nature. Fiberomuscular septa arose from the capsule dividing the gland into lobes. The latter septa gave rise of fiberoelastic septulae, which dividing the lobes into lobules. The prostatic secretory end-pieces were of ubule-alveolar type, which were lined with simple cuboidal epithelium. These cells reacted positively with PAS and alcian blue stains. The intralobular ducts were lined with simple cuboidal epithelium, changed into simple columnar in the interlobular ducts and transitional type in the main excretory ducts, at their opening in the pelvic urethra.