In this study, the whole extract antigen of Sarcocystis fusiformis from buffaloes was assessed for diagnosis of cattle sarcocystosis using ELISA. Prevalence rate of Sarcocystis species among cattle using microscopical examination was 87.69%, while the prevalence rate was 92.31 % by using ELISA. The sensitivity and specificity of ELISA in diagnosis cattle infection were 93.33% and 80%, respectively. We strongly recommended the use of combined microscopical examination and ELISA test for Sarcocystis diagnosis, due to the presence of hidden or microscopic cysts, to avoid human infection of such zoonotic parasite and to control the consequent disease. This study concluded that the use of whole cystic crude antigen of S. fusiformis derived from buffaloes is a reliable mean for diagnosing cattle sarcocystosis