A total of 600 faecal samples from different animal species (200 sheep, 200 cattle, and 200 buffaloes) were screened for the presence of Fasciola gigantica eggs at different localities, Sharkia Province, Egypt. In addition, stool samples were collected from 200 individuals at different hospital laboratories, Sharkia Province. The results revealed that F. gigantica eggs were detected in 97 (16.7%) out of 600 examined animals. The respective overall prevelance of  F. gigantica eggs were 21, 15 and 12.5% in sheep, cattle and buffaloes. The prevalence of  F. gigantica  in females versus males  were (26.09% Vs 14.11%), (20.91% Vs 7.78%) and (18.18% Vs 5.56%) in the examined sheep, cattle and buffaloes, respectively which indicate that F. gigantica was higher in female than male. The infection was higher in the age groups (0-2 years old) in examined sheep, cattle and buffaloes with a percentage of 36, 24 and 21%, respectively. The predominance of F. gigantica infection in relation to season was during winter to be 42% in sheep, 36% in cattle and 30% in buffaloes. The overall prevelance of F. gigantica infection in human was 9 (4.5%) out of 200individualsand it was detected in 7 (5.83%) out of 120 females and 2 (2.5%) out of 80 males. In relation to age groups in human, F. gigantica eggs was 9.72, 1.69 and 1.45% in age individual group of (1-15), (16-30) and ( >30) years old, respectively. The highest prevalence of F. gigantica was detected in human during winter season (14%) while in spring and autumn, it was 2% each. On the other hand, it was not detected in human' stool during summer.