The EMF nowadays is considered as a big source of harmful effects on our life on earth. So, the aim of this experimental study was to investigate the effect of extremely low frequency electromagnetic fields (ELF EMF) on some animal behaviors in rat pups. Laboratory animals with three different ages 2, 4, and 6 weeks were used as control and exposed groups, were placed in a Helmholtz coil electromagnetic field apparatus created for 1 hour and exposure to a 3.5mT, 50Hz. The results showed that exposure of a 50 Hz, 3.5mT electromagnetic field for 1h, has fluctuated effects on some animal behaviors like (activity, inactivity, motion, response to sound, and light, eating, and redness of limbs) in rat pups.