A study was designed to explore the effect of Sanguinarine phytobiotic and Sodium butyrate on growth performance, the hematobiochemical and immunological profile of healthy Broiler Chickens. Ninety (90), one-day old healthy broiler chicks were used in this study and were divided into three equal groups (30 each). Group 1 healthy chicks was kept as control group, group 2 broilers received 2 ml/liter Sodium butyrate and group 3 broilers received 1 gm/10 liter phytobiotic (sangrovite). Administration of drugs in drinking water was continued for 5 successive days, from 19th to 24th day of age. On the first day post administration, two blood samples were collected for hemato-biochemical and immunological analysis. The obtained results revealed that, Broiler chicks received Sodium butyrate and Sanguinarine phytobiotic showed a significant increase in body weight (BW), weight gain (WG), feed consumption (FC) and improvement in feed conversion rate (FCR), total erythrogram and leukogram, phagocytosis and phagocytic index, nitric oxide, HI titre against ND virus, total protein, albumin with non-significant increase in total globulin, a, b, g globulin, albumin/globulin ratio, liver enzymes; aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase (AST ALT and ALP), uric acid and creatinine levels. From the obtained results it could be concluded that, the use of Sodium butyrate and Sanguinarine phytobiotic induced improvement in growth performance, hematobiochemical and immunological profile in healthy broiler chickens and they could be safely used as alternatives to antibiotics growth promoters (AGPs) in the commercial broiler diet to lower the risk of antibiotics residues in the meat and to avoid the antibiotic resistance to the consumers.