Thirty mastitic milk samples were collected from she camels for the isolation and identification of bacteria causing mastitis and to determine their antibiogram against certain antibiotics. Bacteriological examination of mastitic milk samples revealed 18 single isolates (60%)  and 12  mixed isolates (40%). Gentamicin was found to be the highest effective drug against the isolated bacteria than other used drugs. A total of 25 she camels (15 healthy and 10 mastitic) were divided into 5 equal groups receiving gentamicin alone and/or in combination with  isoflupredone acetate. Blood and milk samples were taken from all she camels at 1^st, 7^th and 15^th days post treatment for haematological and biochemical analysis. The results revealed a significant decrease in RBCs count, Hb, PCV%, serum total protein, albumin, globulin, Ca, Ph, Na levels in healthy she camels received gentamicin. While, isoflupredone acetate induced significant increase of WBCs count, AST, ALT, ALP, Ca, Ph, K levels in healthy she camels. Mastitis in she camels lead to significant decrease in RBCs count, Hb, PCV%, albumin, A/G ratio, Ca, Ph, Na, zinc, iron levels and milk production beside no statically difference in K and copper, WBCs, total protein, globulin, AST, ALT and ALP. Hematological and biochemical parameters alterations were returned to nearly normal levels on 10^th day post treatment. It could be concluded that gentamicin and isoflupredone acetate had better results in reducing clinical signs of mastitis and improve adverse effects in she camels.