Ochratoxins are a group of mycotoxins produced as secondary metabolites of several fungi, whichconsists of three members, Ochratoxin A is the most abundant, the most commonly detected member and the most toxic out of the three.  A total of 60 random samples of milk powder marketed in Sharkia Governorate, Egypt (20 each of retail size packages (25gm - 100gm), small packages (2kg)  and bulk packages (25 kg) were collected and  subjected to quantitative detection of Ochratoxin A using immunoaffinty  column method after extraction and then reading by VICAM Fluorometer. Out of 20 examinedretail size packages samples, 50% were contaminated with Ochratoxin A, the minimum was 2.0 ppb, the maximum was 7.6 ppb with a  mean value of 5.14 ± 0.647 ppb, while 65% out of examinedsmall packages were contaminated ( 3.0 to 8.9 ppb)  with a mean value of 6.07 ± 0 .679 ppb, but 80% of  bulk packages were contaminated by range value from 3 to 9.9 ppb with an average of  6.51 ppb. All positive samples were within the permissible limits, according to JECFA, 2001, while according to European commission limits, 2001 20%, 25% and 35% of retail size packages, small packages and bulk packages were within the permissible limits, respectively. But according to United States standard and Egyptian limits, 2010,  50%, 35% and 20% were within the permissible limits respectively.