Rabies antibodies were detected in dog, cat and cattle serum samples and quantified through application of four different methods including Serum neutralization test (SNT), rapid fluorescence focus inhibition test (RFFIT), indirect ELISA and Dot ELISA. The comparison of rabies antibody titers determined in tested samples using RFFIT showed lower than indirect ELISA and SNT. The dot ELISA may have potential applications as a rapid, simple and economical field test in the detection of rabies antibodies but it revealed lower sensitivity and specificity than indirect ELISA and SNT but more than RFFIT. Otherwise, RFFIT needs more time and expensive test.  So, the previous study confirmed that indirect ELISA is the best of choice for detection of rabies antibodies in sera of different animal species because of its highly sensitive, specific and safe laboratory technique.