In this study skin scrab lesions were collected from pigeons showing symptoms of pigeon pox infection from different localities in Egypt. The prepared tissue suspension for inoculated in chorioallentioc membrane (CAM) of specific pathogenic free (SPF) 11day embryonated chicken eggs. The characteristic pock lesions were observed on CAM at the 2^nd passage and. For genetic characterization of the isolated Pigeon pox virus the Polymerase Chain Reaction (PCR) was done from both crust sample and CAM by amplification of P4B gene using the specific forward and reverse primers. The presence of bands of 570 bp is considered positive results.