Antibiotic susceptibility pattern was studied in food poisoning implicated bacteria isolated from a variety of avian origin. Ten salmonella isolates, sixty E. coli isolates, and sixty coagulase positive staphylococci isolates were included in the study.  The phenotypic antibiotic susceptibility patterns of studied isolates revealed that 100% of tested isolates fulfilled the criteria of multidrug resistant bacteria. Conventional PCR was applied to investigate the presence of antibiotic resistance genes, and enterotoxin genes. Testing  the ten Salmonella isolates by PCR  detected stn genes, tetA, and bla^TEM genes with rates of 100%, 60%,and 40% , respectively; Neither aac gene, nor qnRs genes was detected. Fifteen E. coli isolates were tested by PCR, 20%, 100%, 100%, 46.67%, 0%, and 13.33% were positive for aac, tetA, bla^Tem , qnRs , Stx1, and Stx2, respectively. PCR results of fifteen staphylococcus isolates revealed that zero %, 100%, 100% of the tested isolates were positive for aac, tetK, and blaZ, respectively. PCR revealed that none of the tested isolates were positive for sea nor see genes. Meanwhile, 60%, 86.7%, and 20% of staphylococcus isolates were positive for seb, sec, and sed genes, respectively. It could be concluded that poultry, poultry products, and poultry environments could impose public health hazard through disseminating multidrug resistant bacteria with enterotoxigenic potentialities.