In this study, the vaccination of cattle with inactivated bovine ephemeral fever (BEF), live attenuated sheep pox (SP) and inactivated trivalent foot and mouth disease (FMD) vaccines was studied as single and simultaneous application. Twenty one calves were divided into seven groups (three animals each one). The cellular immune response was monitored by MTT based lymphocyte proliferation in peripheral blood mononuclear cell cultures, where it was found that the cellular immune response to FMDV did not affected when simultaneously administered with BEFV, but when administered with SPV only, the cell mediated immune response was earlier with higher levels. On the other hand, there is no significant difference in the determination of antibody levels as measured by (SNT and ELISA) between animal groups vaccinated with FMD and BEF vaccines; while, significant elevation in antibody levels was recorded in the case of vaccination with SP, BEF and FMD vaccines at the same time. So, it can be concluded that simultaneous vaccination of calves with SP, BEF and FMD trivalent vaccines could be considered of applicable benefit.