ManageChatDiscover
7826

Molecular Characterization and Antibiotic Susceptibility of Corynebacterium pseudotuberculosis Isolated from Sheep and Goats Suffering from Caseous Lymphadenitis

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Academic and Pre-clinical Veterinary Sciences (Physiology, Histology, …rology, Parasitology, Pharmacology, Forensic Medicine and Toxicology)

Abstract

 
Caseous lymphadenitis is a highly prevalent worldwide disease of sheep and goats caused by C. pseudotuberculosis, resulting in significant economic losses. In order to investigate the prevalence as well as the molecular characterization of C. pseudotuberculosis and monitoring the antibiotic susceptibility of the isolated strains, a total of 126 pus samples were collected aseptically from 80 sheep and 46 goats suffering from abscessation. The collected samples were subjected to bacteriological examination where the prevalence of C. pseudotuberculosis was 43.8% and 36.9% in diseased sheep and goats, respectively. The antibiotic sensitivity test was carried out using disc diffusion method, most of the isolates were resistant to penicillin (96.2%) and erythromycine (92.3%), highly sensitive to ciprofloxacin (96.2%), amikacin (90.4%) and neomycin (88.5%). Polymerase chain reaction was applied on 30 C. pseudotuberculosis isolates for the amplification and detection of both 16S rRNA and the RNA polymerase β-subunit gene (rpoB) genes to confirm the diagnosis of the isolates. The results revealed that all the tested isolates were positive for both genes. In conclusion, the incorporation of both phenotypic and genotypic characterization of C.pseudotuberculosis is more valuable and accurate for identification of isolates. Moreover, PCR is a fast and specific diagnostic tool used for the genetic analysis of C. pseudotuberculosis.

DOI

10.21608/zvjz.2016.7826

Keywords

Caseous lymphadenitis, C. pseudotuberculosis, 16S rRNA, rpoB

Authors

First Name

Abdelazeem

Last Name

Algammal

MiddleName

-

Affiliation

Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

Email

immunologist222@yahoo.com

City

-

Orcid

-

Volume

44

Article Issue

1

Related Issue

1524

Issue Date

2016-03-01

Receive Date

2018-06-06

Publish Date

2016-03-01

Page Start

1

Page End

8

Print ISSN

1110-1458

Online ISSN

2357-075X

Article File

ZVJZ_Volume 44_Issue 1_Pages 1-8.pdf

PDF . 473.6kB

Link

https://zvjz.journals.ekb.eg/article_7826.html

Detail API

https://zvjz.journals.ekb.eg/service?article_code=7826

Order

1

Type

Original Article

Type Code

601

Publication Type

Journal

Publication Title

Zagazig Veterinary Journal

Publication Link

https://zvjz.journals.ekb.eg/

MainTitle

Molecular Characterization and Antibiotic Susceptibility of Corynebacterium pseudotuberculosis Isolated from Sheep and Goats Suffering from Caseous Lymphadenitis

Details

Type

Article

Created At

22 Jan 2023