The study was conducted to evaluate the role of topical application of either aloe vera gel extract or propolis ointment on wound healing in diabetic and non-diabetic animals. Ninety adult female albino rats weighing (180±200 gm) were divided into 2 groups (A; non-diabetic) and (B; diabetic), each group was further subdivided into 3 subgroups. Group A contains subgroups 1, 2 and 3 but group B contains subgroups 4, 5 and 6.  Subgroups (1, 4) were experimentally punched for induction of skin wound and left untreated for 2 weeks. The wound in both subgroups (2, 5) were treated with Aloe vera extract topically daily. Subgroups (3, 6) skin wound was treated with 10% propolis ointment topically twice daily for 2 weeks. The rats were examined daily during the experiment for any abnormality or mortality rates. Blood samples were collected, besides skin wound specimen from all sacrificed rats on 3, 7, and 14 dpi for cellular immune response and histopathological examination, respectively. Both treated rats mainly (subgroup 2, 3 and 6) were responded to treatment with rapid healing which begin from 7 dpi compared to (subgroups 1, 5) which showed delayed healing. However, subgroup (4) was difficult to response to recovery until 14 dpi. It could be concluded that both propolis and Aloe vera accelerated the healing process in both diabetic and non-diabetic wounded animals.