7890

Methodology of Using eCG or FSH Regimens for Multiple Ovulations and Embryo Recovery by Assisted Reproductive Technologies in Sheep

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Clinical Science, Surgery and Veterinary Diseases (Pathology, Clinical…ltry Diseases, Fish Diseases and Management, Surgery, Theriogenology)

Abstract

This study aimed to profit the assisted reproductive technologies in sheep presented two techniques for multiple ovulations and embryo recovery. Multiparous ewes of Egyptian Barki (N=15) and Australian Dorper (N=20) were used in the first and second techniques, respectively. Technique I was performed in Faculty of Veterinary Medicine, Sadat city, Menufia province, Egypt during the period from August to February, while technique II in Werribee, Victoria, Melbourne City Australia during the period from March to April. The technique I (20-21 days) based on using intravaginal progesterone (P4)-sponges for fourteen days, equine chorionic gonadotropin and flushing of embryos under local anesthesia 5 days after mating. However, the technique II (18 days) based on using intravaginal P4-controlled internal drug release silicon devices for twelve days with six injections of the follicle-stimulating hormone (Folltropin; FSH), eCG and surgical flushing of embryos under general anesthesia four days after laparoscopic intrauterine insemination. The recovery rate of the harvested morulae and blastocysts in relation to the developed corpora lutea were 38.2 and 24.4 % in the technique I, respectively, while they were 40.8 and 25.8 % in the technique II, respectively. Moreover, the percent of unfertilized oocytes and lost recovered structures, those of early embryonic death absorbed or lost during flushing in the technique I were 18.2 and 31.6 %, respectively, while they were 7.3 and 13.7 % in the technique II, respectively. In conclusion, the two presented techniques showed variable results of recovered embryos, but the harvested embryos produced by ewes younger than 6 years old were higher than 38 %.

DOI

10.21608/zvjz.2017.7890

Keywords

Barki, embryo, MOET, Dorper, sheep, superovulation

Authors

First Name

Ahmed

Last Name

Ahmed

MiddleName

E.

Affiliation

Theriogenology Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt

Email

ezzat_29@yahoo.com

City

-

Orcid

-

First Name

Tamer

Last Name

Genedy

MiddleName

M.

Affiliation

Theriogenology Department, Faculty of Veterinary Medicine, Sadat City University, 32897, Sadat, Egypt

Email

-

City

-

Orcid

-

First Name

Hassan

Last Name

Ahmed

MiddleName

-

Affiliation

Physiology Department, Faculty of Veterinary Medicine, South Valley University, 83523, Qena City, Egypt

Email

-

City

-

Orcid

-

Volume

45

Article Issue

2

Related Issue

1520

Issue Date

2017-06-01

Receive Date

2018-05-10

Publish Date

2017-06-01

Page Start

177

Page End

187

Print ISSN

1110-1458

Online ISSN

2357-075X

Link

https://zvjz.journals.ekb.eg/article_7890.html

Detail API

https://zvjz.journals.ekb.eg/service?article_code=7890

Order

9

Type

Original Article

Type Code

601

Publication Type

Journal

Publication Title

Zagazig Veterinary Journal

Publication Link

https://zvjz.journals.ekb.eg/

MainTitle

Methodology of Using eCG or FSH Regimens for Multiple Ovulations and Embryo Recovery by Assisted Reproductive Technologies in Sheep

Details

Type

Article

Created At

22 Jan 2023