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7886

Laboratory Detection and Neutralizing Activity of Exocellular AmpC β-lactamases by Anti bla-CMY

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

Clinical Science, Surgery and Veterinary Diseases (Pathology, Clinical…ltry Diseases, Fish Diseases and Management, Surgery, Theriogenology)

Abstract

Detection of AmpC β-lactamases (AmpC-bls) is important for infection control purposes and therapeutic options. Here, we provided a diagnostic anti β-lactamase neutralization test (bla-NT); modified from broth microdilution (BM) for the detection of bls-AmpC, CMY, in multidrug resistant Escherichia coli and Klebsiella pneumoniae. Anti-bla neutralizing activity against these two bacteria was tested. Anti bla-CMY was prepared in rabbits and used in: bla-NT; investigating effect on bacterial colony forming unit (CFU); and in ELISA. In bla-NT, the anti-bla-CMY neutralized exocellular bls produced by the tested bacterial strains and resulted in an increase in the bacterial sensitivity to the tested antimicrobials and reduction in minimum inhibitory concentration.  Interestingly, the anti-bla-CMY decreased the CFU and its morphology when added to the tested bacteria. ELISA-OD was significantly correlated with the drop in minimum inhibitory concentration and CFU counts at P-value ≤ 0.05 and 0.01, respectively. It could be concluded that, bla-NT could detect bls-AmpC and run parallel to BM in microbiology laboratory. Investigations are running to develop the test for quantitative detection of bls-AmpC.

DOI

10.21608/zvjz.2017.7886

Authors

First Name

Adel

Last Name

Attia

MiddleName

M.

Affiliation

Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt

Email

-

City

-

Orcid

-

First Name

Eman

Last Name

Fatah

MiddleName

A.

Affiliation

Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt

Email

-

City

-

Orcid

-

First Name

Gamal

Last Name

El-mowalid

MiddleName

A.

Affiliation

Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt

Email

gelmowalid@yahoo.com

City

-

Orcid

-

Volume

45

Article Issue

2

Related Issue

1520

Issue Date

2017-06-01

Receive Date

2017-03-18

Publish Date

2017-06-01

Page Start

134

Page End

142

Print ISSN

1110-1458

Online ISSN

2357-075X

Article File

ZVJZ_Volume 45_Issue 2_Pages 134-142.pdf

PDF . 304.7kB

Link

https://zvjz.journals.ekb.eg/article_7886.html

Detail API

https://zvjz.journals.ekb.eg/service?article_code=7886

Order

5

Type

Original Article

Type Code

601

Publication Type

Journal

Publication Title

Zagazig Veterinary Journal

Publication Link

https://zvjz.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023