The research was conducted to study the nutritional evaluation of pumpkin seed and the effect of addition of pumpkin seeds powder and oil on blood level of glucose and fat in diabetic rats. Firstly , the nutritional composition and antioxidant activity of pumpkin seeds powder were determined. Secondly, a total of 60 rats were randomized into 6 groups as follows: Group 1: Normal control; Group 2: Diabetic control; Group 3: Diabetics fed with pumpkin seeds powder (1%); Group 4: Diabetics fed with pumpkin seeds powder (3%) ; Group 5: Diabetics fed with pumpkin seed oil (1%) ; Group 6: Diabetics fed with pumpkin seed oil (3%). The rats were injected with alloxan 150 mg / kg BW for induction the diabetes in rats until their blood glucose level reached more than 200 mg / dL. The results showed that pumpkin seeds rich in carbohydrates, protein, crude fiber and crude oil, many unsaturated fatty acids, especially linoleic and oleic acids. It is also a rich source of antioxidants. In addition to that glucose, glycated hemoglobin, cholesterol, triglycerides, LDL,VLDL and lipid peroxidation were significantly increased (P