Extraction of M. oleifera seed using methanol and petroleum ether solvent has an effect on T. urticae, Results showed that polar solvent more effective than non-polar where LC50 after 5 days of treatment was 0.95 ppm at methanol solvent, where it was 1.74 ppm at petroleum ether solvent after 5 days of treatment. At different values of pH, the results showed that LC50 was 7.37 ppm at pH (4), 5.19 ppm at pH (7) while it was 3.19 ppm at pH (9). There are different compounds in seeds of Moringa which have effect on T. urticae, the most component on seeds was Oleic acids, Hexadeconic acid, and Octadeconic acid. The chemical constitute of M. oleifera with methanolic extract at different pH has a different compounds which responsible for the effect on mites like oleic acid, hexadecanoic acid, and octadecenoic acid.