The use of exogenous hormones to induce final oocyte maturation, ovulation, and spawning has become
commonplace in the reproductive protocols for many species. In the present study, African catfish (Clarias gariepinus)
were monthly collected from January to December 2011 for studying their monthly reproductive biology. Also,
hormonal stimulation was done using ovaprim, human chorionic gonadotropin (hCG), and salmon gonadotropin
releasing hormone analog (GnRHa). However, fish (337.2 – 718.0 g) were divided into seven groups (each group
contained four females and four males) and injected into the dorsal musculature by 0.4, 0.5 ml kg -1 Ovaprim, 1000,
3000 IU kg -1 hCG, and 0.75, 1 ml kg -1 GnRHa. The control group was injected by 0.9% saline. At the dosage tested,
gonado-somatic index (GSI), absolute fecundity, ovulation index, egg diameter, fertilization rate were significantly
affected in all stimulated groups. It was found that the hormonal treatments are a useful method for commercial African
catfish breeders to ensure the continuity of access larvae. However, the best reproductive performance was obtained at
0.5 ml kg -1 ovaprim, 3000 IU kg -1 hCG, and 0.75 ml kg -1 GnRHa.