OBJECTIVES: To study the effect of oral administration of grape seed oil (GSO)
against carbontetrachloride (CCl4)-induced hepatotoxicity in rat Liver damage was
induced in male Wistar rats (150-200 g) by administering CCl4 (0.5 ml/kg, i.p.) once
per day for 7 days and the extent of damage was studied by assessing biochemical
parameters such as alanine aminotransferase (ALT), aspartate aminotransferase
(AST), and alkaline phosphatase (ALP) in serum and concentrations of glutathione
(GSH), superoxide dismutase (SOD), and total protein (TP) in liver. The effect of co-administration of GSO (3.7 g/kg, orally) on the above parameters was further
investigated and compared with a vitamin E (100 mg/kg, orally) treated group.
RESULTS: Oral administration of GSO (3.7 g/kg, body weight orally) for 7 days
resulted in a significant reduction in serum AST, ALT, and ALP levels and significant
improvement in glutathione, SOD, and TP, when compared with CCl4 damaged rats.
The antioxidant effect of GSO at 3.7 g/kg for 7 days was found to be comparable with
vitamin E (100 mg/kg, orally) in CCl4-treated rats.
CONCLUSION: The GSO has protected the liver from CCl4 damage. Probable
mechanism of action may be due to the protection against oxidative damage produced
by CCl4.