7,12-dimethylbenz(a) anthracene (DMBA)has an oxidative damaging effects and
grape seed oil (GSO), among its protective mechanisms is the antioxidant properties.
Objective: to investigate the hepatorenal protective effects of dietary GSO against
liver and renal cytotoxicity induced by DMBA in the rats. Materials and methods:
The experimental animals were divided into four groups, the first group was
untreated and used as control, the second was treated for 2 weeks with DMBA alone,
the third was treated with DMBA plus GSO for 2 weeks, and the fourth group was
treated with GSO after DMBA for 2 weeks. Serum liver enzymes, lactate
dehydrogenase (LDH), glutamic pyruvic acid transaminase (GPT), glutamic
oxaloacetic acid transaminase (GOT), and total plasma proteins (TPS), albumin and
globulins were chosen to assess liver function. Urea and creatinine are measured as
some of renal function tests. Results: In comparison with control group, DMBA alone
significantly increased the serum level of liver GOT,GPT and LDH enzymes and urea
and creatinine levels, while total proteins, albumin and globulin was decreased. GSO
plus DMBA had decreased significantly, GOT, GPT and LDH and urea while,
creatinine was insignificantly changed, also, it had increased total proteins, albumin
and globulin when compared with rats subjected to DMBA alone. Administration of
GSO weather plus or after DMBA had increased albumin, but, it is significantly lower
than in control rats. Conclusion: Treatment with GSO plus DMBA significantly
decreases the changes in some hepatic and renal functions induced by DMBA in rats